Email: info@neweastbio.com   |  Call: 6109452007

Rap1-GTP

Anti-Rap1-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26912

   Size:   30 μl

In Stock

            Product Description          
Rap1-GTP
Cat. #:   26912
Size:  30 µL
Gene Symbol:  RAP1
Description:   Anti-Rap1-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
Immunogen:   Recombinant full length protein of active Rap1.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rap1-GTP monoclonal antibody recognizes active Rap1 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   Rap1 Activation Assay.Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rap1-GTP monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).
          Related Products          
          Publications          
01. Loss of TTC17 promotes breast cancer metastasis through RAP1/CDC42 signaling and sensitizes it to rapamycin and paclitaxel
Cell Biosci. 2023   PMID: 36895029
02. 25-hydroxycholesterol inhibits human papillomavirus infection in cervical epithelial cells by perturbing cytoskeletal remodeling
J Med Virol. 2023   PMID: 37254637
03. EPAC Regulates Melanoma Growth by Stimulating mTORC1 Signaling and Loss of EPAC Signaling Dependence Correlates with Melanoma Progression
Mol Cancer Res. 2022  PMID: 35834616
04. Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity
Front Cell Dev Biol. 2022  PMID: 35372328
05. Actin-binding protein, IQGAP1, regulates LPS-induced RPMVECs hyperpermeability and ICAM-1 upregulation via Rap1/Src signalling pathway
Cell Signal. 2021  PMID: 34147590
06. Active Rap1-mediated inhibition of choroidal neovascularization requires interactions with IQGAP1 in choroidal endothelial cells
FASEB J. 2021  PMID: 34166557
07. Natural Killer Cell Transcript 4 promotes the development of Sjögren’s Syndrome via activation of Rap1 on B cells
J Autoimmun. 2021  PMID:  33087256
08. Activation of the Small GTPase Rap1 Inhibits Choroidal Neovascularization by Regulating Cell Junctions and ROS Generation in Rats
Curr Eye Res. 2018  PMID: 29601231
09. Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIa and Rap1
Sci Rep. 2017  PMID: 29042611
10. Opposing effects of actin signaling and LFA-1 on establishing the affinity threshold for inducing effector T-cell responses in mice
Eur J Immunol. 2016  PMID: 27188212
11. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization
Mol Ther Methods Clin Dev. 2016  PMID: 27606349
12. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming
Mol Ther. 2015  PMID: 25666918
13. Activation of Rap1 inhibits NADPH oxidase-dependent ROS generation in retinal pigment epithelium and reduces choroidal neovascularization
FASEB J. 2014  PMID: 24043260
14. Opposing HDAC4 nuclear fluxes due to phosphorylation by ß-adrenergic activated protein kinase A or by activity or Epac activated CaMKII in skeletal muscle fibres
J Physiol. 2013  PMID: 23652597
15. Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo
PLoS One, 2013  PMID: 24039860