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Ras(G12D)

Anti-Ras(G12D) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26036

   Size:   100 μl

In Stock

            Product Description          
Ras(G12D)
Cat. #:  26036
Gene Symbol:  RAS(G12D)
Description:   Anti-Ras(G12D) Mouse Monoclonal Antibody
Background:   Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin
Applications:    ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:100-1:3000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype: IgG1, IgG2a, IgG2b
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant Ras WT and G12D proteins. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G12D (lane 2, Cat # 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody (Cat # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021). Immunofluorescence:   image.png Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(WT) plasmid (left column) or pCDNA3-GFP Ras(G12D) plasmid (right column), then fixed and stained with anti-Ras(G12D) monoclonal antibody (Cat. # 26036).
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01. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer
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02. Membrane Anchorage-Induced (MAGIC) Knockdown of Non-synonymous Point Mutations
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03. The GSK3 kinase and LZTR1 protein regulate the stability of Ras family proteins and the proliferation of pancreatic cancer cells
Neoplasia. 2022  PMID: 35114566
04. SF3B1 promotes tumor malignancy through splicing-independent activation of HIF1à
bioRxiv. 2020  PMID: 
05. Selective targeting of the oncogenic KRAS G12S mutant allele by CRISPR/Cas9 induces efficient tumor regression
Theranostics. 2020  PMID: 32308773
06. Transcriptional and metabolic rewiring of colorectal cancer cells expressing the oncogenic KRASG13D mutation
Br J Cancer. 2019  PMID: 31133691
07. A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal
Dis Model Mech. 2019  PMID: 30910991
08. Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer
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09. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
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10. CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A
Oncogene. 2018  PMID: 28892048
11. KRASG12D and TP53R167H Cooperate to Induce Pancreatic Ductal Adenocarcinoma in Sus scrofa Pigs
Sci Rep. 2018  PMID: 30135483
12. Antibody-mediated depletion of protein variant expression in living cells (Protein interference)
biorxiv. 2017  PMID: 
13. TGFá Signaling in the Pancreatic Tumor Microenvironment Promotes Fibrosis and Immune Evasion to Facilitate Tumorigenesis
Cancer Res. 2016   PMID: 26980767
14. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Ther. 2014  PMID: 25077772