Catalog Number:  21002

Gene Symbol:  Orai1

Description:   Anti-Orai1 Rabbit Polyclonal Antibody (Orai1-L2)

Background:   Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Immunogen:   A peptide from the extracellular loop 2 region of human Orai1

Applications:   ELISA, WB

Recommended Dilutions:    ELISA:      1:1000-1:5000, WB:          1:1000-1:2000, IHC:          1:100-1:200

Concentration:

Host:   Rabbit

Format:   Liquid

Clonality:   Polyclonal

Isotype:   IgG

Purity:   Immunogen affinity purified

Storage buffer:

Preservative:  0.01% sodium azide

Constituents:   50mM NaHCO3-Glycine, 0.15M NaCl,, pH7.4

Species Reactivity:  Orai1-L2 antibody recognizes Orai1 from human.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (A) or presence (B) of antigen peptide.

Publications:
1.   Orai1 and STIM1 are critical for breast tumor cell migration and metastasis    S Yang, JJ Zhang, XY Huang - Cancer cell, 2009
2.   Up-regulation of Orai1 in murine allergic rhinitis   L Lin, C Zheng, L Zhang, C Da, K Zhao - Histochemistry and Cell Biology