Catalog Number:  26904

Gene Symbol:  RHOA

Description:   Anti-Active RhoA Mouse Monoclonal Antibody

Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.

Immunogen:   Recombinant full length protein of active RhoA

Applications:   IP, IHC

Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;                                                    1:100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

View IHC/IF protocol

Immunoprecipitation/Western Blot:

IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPgS (lane 2). These proteins were immunoprecipitated with anti-active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26008).

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