Catalog Number:  26002-1

Gene Symbol:

Description:   Anti-cAMP Mouse Monoclonal Antibody

Background:   cAMP is a ubiquitous second messenger mediating cellular responses to various exogenous and endogenous signaling molecules. cAMP regulates physiological processes by activating protein kinases, gating specific ion channels, modulating cellular cyclic nucleotide concentrations through phosphodiesterases, and activating Epac (exchange protein directly activated by cAMP). The conversion of ATP to cAMP is catalyzed by adenylyl cyclases (ACs). The major family of ACs in mammals is the transmembrane ACs which have nine isoforms and could be activated by G protein Gs and/or Ca2+/calmodulin. There is also one soluble AC which could be modulated by bicarbonate and/or Ca2+.

Immunogen:   cAMP

Applications:   ELISA, WB, IHC

Recommended Dilutions:   ELISA 1:1000-1:5000, WB 1:1000-1:2000

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Typical Standard Curves

Sensitivity   Acetylated Version Mean OD for Bo = 0.565 ± 0.007 Mean OD for Standard #6 = 0.500 ±0.006 Delta Optical Density (0-0.08 pmol/mL) = 0.065 2 SD's of the Zero Standard = 0.014 Sensitivity =(0.014/0.065)*0.08 pmol/mL=17 fmol/mL