Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. Sar1 is invloved in membrane trafficking, and regulates the assembly and disassembly of COPII coats.
Currently there is no direct assay to measure the activation of Sar1 GTPases.
NewEast Biosciences Sar1 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Sar1-GTP, but not Sar1-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti-Sar1-GTP monoclonal antibodies can also be used to monitor the activation of Sar1 in cells and in tissues by immunohistochemistry.
NewEast Biosciences Sar1 Activation Assay Kit provides a simple and fast method to monitor the activation of Sar1. Each kit provides sufficient quantities to perform 20 assays.
Assay Principle
NewEast Biosciences Sar1 Activation Assay Kit bases on the configuration-specific anti-Sar1-GTP monoclonal antibody to measure the active Sar1-GTP levels, either from cell extracts or from in vitro GTP?S loading Sar1 activation assays. Briefly, anti-active Sar1 mouse monoclonal antibody will be incubated with cell lysates containing Sar1-GTP. The bound active Sar1 will then be pulled down by protein A/G agarose. The precipitated active Sar1 will be detected by immunoblot analysis using anti-Sar1 rabbit polyclonal antibody.
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