NewEast Biosciences Rab5C Activation Assay Kit bases on the configuration-specific anti-Rab5-GTP monoclonal antibody to measure the active Rab5C-GTP levels, either from cell extracts or from in vitro GTP?S loading Rab5C activation assays. Briefly, anti-active Rab5 mouse monoclonal antibody will be incubated with cell lysates containing Rab5-GTP. The bound active Rab5 will then be pulled down by protein A/G agarose. The precipitated active Rab5C will be detected by immunoblot analysis using anti- Rab5C specific rabbit polyclonal antibody.