|Place of Origin||USA|
|Technical Information||View technical data|
Arl3 Protein 
|Catalog Number: 10152|
|Product Name: Arl3 Protein|
|Synonyms: ADP-ribosylation factor-like 3, ARFL3|
|Source: Human, recombinant full length, His6-tag|
|Expression Host: E. coli|
|Molecular Weight: 20 kDa|
|Purity: >95% by SDS-PAGE|
|Introduction: Arl3 belongs to the member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases. Arl3 is also a member of the ADP-ribosylation factor family of GTP-binding proteins. Arl3 binds guanine nucleotides but lacks ADP-ribosylation factor activity.|
|Amino Acid Sequence (1-182)|
|Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.|
|Physical Appearance (form): White or clear|
|Concentration: 1 mg/mL|
|Preparation Instructions: |
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arl3 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
1. Cavenagh, M. M. et al., J. Biol. Chem. 269: 18937-18942, 1994.
2. Grayson, C. et al., Hum. Molec. Genet. 11: 3065-3074, 2002.
3. Kim, H.-S. Cytogenet. Cell Genet. 83: 246-only, 1998.
4. Schrick, J. JAm. J. Path. 168: 1288-1298, 2006.
5. Veltel, S. et al., Nature Struct. Molec. Biol. 15: 373-380, 2008.