| Peer Reviews |
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Publications
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| Activation Assay Kits |
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| Antibodies for GTP-bound G Proteins |
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| cAMP/cGMP EIA Kits without Acetylation |
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| Biomarkers |
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| Monoclonal Antibodies |
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| Protocols |
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Protocol for IF/IHC
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Protocol for IF/IHC
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- Rinse cells briefly in PBS (37°C, as quickly as possible).
- Aspirate PBS, cover cells with 300~500 µl 4% formaldehyde in PBS.
- Allow cells to fix for 15 minutes at room temperature.
- Aspirate fixative, rinse three times in PBS for 1 minute each.
- Block specimen in 5% FBS in PBS/Triton for 60 minutes.
- While blocking, prepare primary antibody by diluting 100 X in PBS/Triton (1:100 dilution).
(You will need 200~300 µl per coverslip.)
- Aspirate blocking solution, apply diluted primary antibody.
NOTE: For double-labeling, prepare a cocktail of mouse and rabbit primary antibodies at their appropriate dilutions in PBS/Triton.
- Incubate overnight at 4°C.
- Rinse 3X in PBS for 5 minutes each.
- Incubate with fluorochrome-conjugated secondary antibody diluted in PBS/Triton for 1-2 hours at room temperature in dark.
NOTE: For double-labeling, prepare a cocktail of fluorochrome-conjugated anti-mouse and anti-rabbit primary antibodies at their appropriate dilutions in PBS/Triton.
- Rinse 3X in PBS for 5 minutes each.
- Examine specimens immediately using appropriate excitation wavelength, depending on fluorochrome for best results or store at 4°C in dark.
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PBS/Triton: 1X PBS/0.3% Triton X-100
 Download Procedure
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| IHC/IF Publications | |
1. Physiological Activation of Synaptic Rac> PAK (p-21 Activated Kinase) Signaling Is Defective in a Mouse Model of Fragile X Syndrome
LY Chen, CS Rex, AH Babayan, EA Kramar, G Lynch, CM Gall and JC Lauterborn
The Journal of Neuroscience, 2010 | |
2. Antioxidants Counteract Nicotine and Promote Migration via RacGTP in Oral Fibroblast Cells
S Miguel, L Opperman, E Allen, J Zielinski and K Svoboda
Journal of Periodontology, 2010 | |
3. K-Ras Mediated Murine Epidermal Tumorigenesis Is Dependent upon and Associated with Elevated Rac1 Activity
MS Samuel, FC Lourenco and MF Olson
OPLoS ONE, 2011 - dx.plos.org | |
| View IHC/IF Images
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Active Gα13-GTP (26902) on MEF cells
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Active Cdc42-GTP (26905) on MS1 cells
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Active Rac1-GTP (26903) on brain tissue sections
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