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Antibodies for GTP-bound G Proteins

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Anti Rheb-GTP Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26910
ImmunogenMouse
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Rheb-GTP [26910]
    [30 µL]

    Catalog Number:  26910
    Gene Symbol:  RHEB
    Description:   Anti Active Rheb Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
    Immunogen:   Recombinant full length protein of active Rheb.
    Applications:   IP, IHC
    Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
                                                  1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgM
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rheb antibody recognizes active Rheb of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rheb monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).



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    Publications:
    1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
        Cancer Res. 2014 Feb 15;74(4):1067-78
    2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
        PLoS One. 2013 Dec 31;8(12):e83941