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Antibodies for GTP-bound G Proteins

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Anti Ras -GTP Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26909
ImmunogenMouse
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Ras-GTP [26909]
    [30 ul]


    Catalog Number:  26909
    Gene Symbol:  RAS
    Description:   Anti Active Ras Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active H-Ras
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  

    IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # 26014).


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    Publications:
    1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
        Gene Therapy 21, 888-896 (October 2014)