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Antibodies for GTP-bound G Proteins

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Anti Rap1-GTP Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26912
ImmunogenMouse
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Rap1-GTP [26912]
    [30 µL]


    Catalog Number:  26912
    Gene Symbol:  RAP1
    Description:   Anti Active Rap1 Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
    Immunogen:   Recombinant full length protein of active Rap1.
    Applications:   IP, IHC
    Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).



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    Publications:
    1.  Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
        PLoS One. 2013 Sep 10;8(9):e73070