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Antibodies for GTP-bound G Proteins


Anti Ran-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26915
Size30 μl
Technical InformationView technical data
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  • Summary

    Active Ran-GTP [26915]
    [30 µL]

    Catalog Number:  26915
    Gene Symbol:  RAN
    Description:   Anti Active Ran Mouse Monoclonal Antibody
    Background:   Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
    Immunogen:   Recombinant full length protein of active Ran.
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Ran antibody recognizes active Ran of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Ran activation assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Ran monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody.

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    1.  NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
        Nucleic Acids Res. 2012 Sep 1;40(17):8484-98