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Antibodies for GTP-bound G Proteins


Anti Rab4A-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26921
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Rab4A-GTP [26921]
    [30 µL]

    Catalog Number:  26921
    Gene Symbol:  RAB4
    Description:   Anti Active Rab4 Mouse Monoclonal Antibody
    Background:   Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.
    Immunogen:   Recombinant full length protein of active Rab4A.
    Applications:   IP, IHC
    Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
    1:50~100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S(lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.
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    1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
        Hepatology Volume 61, Issue 1, pages 361–374, January 2015
    2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
        Endocrinology. 2014 Sep;155(9):3315-28