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Antibodies for GTP-bound G Proteins

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Anti Rab35-GTP Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26922
ImmunogenMouse
Size30 μl
Technical InformationView technical data
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  • Summary

    Active Rab35-GTP [26922]
    [30 µL]

    Catalog Number:  26922
    Gene Symbol:  RAB35
    Description:   Anti Active Rab35 Mouse Monoclonal Antibody
    Background:   The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
    Immunogen:   Recombinant full length protein of active Rab35
    Applications:   IP, IHC, IF
    Recommended Dilutions:   
    1 µg for ~2 mg total cellular proteins for IP 
    1:100 dilution for IHC, IF
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).


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    Publications:
    1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
        Hepatology Volume 61, Issue 1, pages 361–374, January 2015
    2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
        Endocrinology. 2014 Sep;155(9):3315-28