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Antibodies for GTP-bound G Proteins


Anti Rab11-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26919
Size30 μl
Technical InformationView technical data
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  • Summary

    Active Rab11-GTP [26919]
    [30 µL]

    Catalog Number:  26919
    Gene Symbol:  RAB11
    Description:   Anti Active Rab11 Mouse Monoclonal Antibody
    Background:   Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
    Immunogen:   Recombinant full length protein of active Rab11
    Applications:   IP, IHC
    Recommended Dilutions:   IP: 1 μg for 1~2 mg total cellular proteins
    IHC: 1:50~100 dilution
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2b
    Purity:    Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rab11 antibody recognizes active Rab11 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-active Rab11 monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).

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    1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
        Hepatology Volume 61, Issue 1, pages 361–374, January 2015
    2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
        Endocrinology. 2014 Sep;155(9):3315-28