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Antibodies for GTP-bound G Proteins


Anti Sar1-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26916
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Sar1-GTP [26916] 
    [30 µL]

    Catalog Number:  26916
    Gene Symbol:  SAR1
    Description:   Anti Active Sar1 Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.
    Immunogen:   Recombinant full length protein of active Sar1a.
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).

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