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Antibodies for GTP-bound G Proteins


Anti Gαo-GTP Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26907
Size30 μl
Technical InformationView technical data
  • Data Sheet
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  • Summary

    Active Gαo GTP [26907]
    [30 µL]

    Catalog Number:  26907
    Gene Symbol:  Gnao
    Description:   Anti Active Gαo Mouse Monoclonal Antibody
    Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
    Immunogen:   Recombinant full length protein of active Gαo
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG1
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    o activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαopolyclonal antibody (Cat. No. 21015).

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