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Anti β catenin Rabbit Polyclonal Antibody

Price$219.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number21282
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    β-catenin [21282]
    [100 µL]

    Catalog Number:  21282
    Gene Symbol:  CTNNB1, CTNNB
    Description:   Anti β catenin Rabbit Polyclonal Antibody
    Background:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
    Immunogen:    A synthetic peptide from the internal region of β-catenin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:2000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   
    Purity:    Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes β-catenin from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #21282).
    Immunohistochemistry:  
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.


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