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Antibodies for Proto-oncogene

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Anti ErbB2 Rabbit Polyclonal Antibody

Price$219.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number21202
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    ERBB2 [21202]
    [100 µL]

    Catalog Number:  21202
    Gene Symbol:  ERBB2/HER2/Neu
    Description:   Anti ErbB2 Rabbit Polyclonal Antibody
    Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.
    Immunogen:   A synthetic peptide from the central of ErbB2 protein.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:500-1:5000 
    WB: 1:200-1:2000 
    IHC: 1:20-1:200
    Concentration:   0.2 mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   
    Purity:   Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes ErbB2 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
    Download Datasheet:  
    Western blot:  
    Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti ErbB2 rabbit polyclonal antibody (Cat. #21202).
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.


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