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Publications
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| Activation Assay Kits |
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| Antibodies for GTP-bound G Proteins |
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| cAMP/cGMP EIA Kits without Acetylation |
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| Biomarkers |
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| Monoclonal Antibodies |
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| Protocols |
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Protocol for IF/IHC
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| Active Cdc42-GTP 26905
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| The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN(3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.
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| Negative Control
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| | Protocols |
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Protocol for IF/IHC
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| Antibodies for Mutated Oncogenes |
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| Polyclonal Antibodies |
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| Purified G Proteins |
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