A Helping Hand for Your Research    Advanced Search Cart | Checkout |                       Activation Assay Kits Gαi Ras Gα13 Rac1 Gαs RhoA Gαo Cdc42 Gαz Antibodies for Active G Proteins Gαi-GTP Ras-GTP Gα13-GTP Rac1-GTP Gαs-GTP RhoA-GTP Gαo-GTP Cdc42-GTP Gαz-GTP EIA Kits cAMP - No Acetylation cGMP - No Acetylation Monoclonal Antibodies Gαi Ras Gα13 Rac1 Gαs RhoA Gαo Cdc42 Gαz cAMP cGMP β1-Adrenergic Receptor Polyclonal Antibodies Gαi Gαq Gα13 Gαz Gαs Rac1 Gαo RhoA Gα12 Cdc42 Orai1-L1 Orai1-L2 Protocols Protocol for IF/IHC Custom Antibody Anti-active G Protein Antibodies Peer Reviews Publications Order Phone:  610-945-2007 Fax     :  610-945-2008 Email  :  sale@neweastbio.com Active Cdc42-GTP 26905    The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN(3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.    Negative Control Copyright @ 2006 NewEast Biosciences All rights reserved