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Anti BRAF(G469E) Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26196
ImmunogenMouse
Size100ul
Technical InformationView technical data
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  • Summary

    B-RAF(G469E) [26196]
    [100ul]


    Catalog Number:  26196
    Gene Symbol:  BRAF, BRAF1, RAFB1
    Description:   Anti BRAF(G469E) Mouse Monoclonal Antibody
    Background:    BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
    Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes G469E mutant, but not wild-type BRAF of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant BRAF (G469E) and wildtype proteins. Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti BRAF(G469E) monoclonal antibody (Cat. #26196).
    Immunofluorescence:  
    Immunofluorescence of cells expressing BRAF proteins with Anti BRAF(G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti BRAF(G469E) monoclonal antibody (Cat. #26196).



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    Publications:
    1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
        Mod Pathol. 2013 Mar;26(3):414-20.
    2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
        Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
    3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
        human pathology. February 2015Volume 46, Issue 2, Pages 169–175
    4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
        human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
    5.  BRAF V600E Mutation-Specific Antibody: A Review
        Seminars in Diagnostic Pathology


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