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Antibodies for Mutated Oncogene

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Anti BRaf(V600D) Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26184
ImmunogenMouse
Size100ul
Technical InformationView technical data
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  • Summary

    BRaf(V600D) [26184]
    [100 µL]


    Catalog Number:  26184
    Gene Symbol:  BRAF, BRAF1, RAFB1
    Description:   Anti BRaf(V600D) Mouse Monoclonal Antibody
    Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
    Immunogen:   A synthetic peptide from the internal region of BRaf(V600D), human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA 1:1000-1:5000 
    WB 1:500-1:2000 
    IHC 1:100-1:200
    Concentration:    0.5 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes B-Raf V600D, but not wild-type B-Raf proteins from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti BRaf(V600D) monoclonal antibody (Cat. # 26184).
    Immunofluorescence:   
    Immunofluorescence of cells expressing B-Raf proteins with Anti BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(V600D) plasmid (right column), then fixed and stained with Anti BRaf(V600D) monoclonal antibody (Cat. #26184).


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    Publications:
    1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
        Mod Pathol. 2013 Mar;26(3):414-20.
    2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
        Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
    3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
        human pathology. February 2015Volume 46, Issue 2, Pages 169–175
    4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
        human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
    5.  BRAF V600E Mutation-Specific Antibody: A Review
        Seminars in Diagnostic Pathology


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