A Helping Hand for Your Research

Phone: 610-945-2007


Antibodies for Mutated Oncogene


Anti RHEB(Y35N) Mouse Monoclonal Antibody

Place of OriginUSA
Catalog Number26338
Size100 μl
Technical InformationView technical data
  • Data Sheet
  • Reference
Purchase by sweeping
  • Summary

    RHEB(Y35N) [26338]

    Catalog Number:  26338
    Gene Symbol:  RHEB
    Description:   Anti RHEB(Y35N) Mouse Monoclonal Antibody
    Background:   RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.
    Immunogen:   A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes Y35N mutant, but not wild-type RHEB of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant RHEB(Y35N) and wildtype proteins. Purified His-tagged RHEB(Y35N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338).
    Immunofluorescence of cells expressing RHEB proteins with Anti RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP-RHEB (WT) plasmid (left column) or pCDNA3-GFP-RHEB(Y35N) plasmid (right column), then fixed and stained with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338).

    Related Products
    81201RheB Activation Assay Kit 20 assays $737   
    26910Active RheB-GTP Monoclonal Antibody 30 μL $495   
    26015Anti-RheB Mouse Monoclonal Antibody 100 μL $239   
    21098Anti-RheB Rabbit Polyclonal Antibody 100 μL $219   
    26338Anti RHEB(Y35N) Mouse Monoclonal Antibody 100 μL $495  

    1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
        Cancer Res. 2014 Feb 15;74(4):1067-78
    2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
        PLoS One. 2013 Dec 31;8(12):e83941

      no data