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Anti BRaf(V600E) Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26039
ImmunogenMouse
Size100ul
Technical InformationView technical data
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  • Summary

    BRaf(V600E) [26039]
    [100 µL]


    Catalog Number:  26039
    Gene Symbol:  BRaf
    Description:   Anti BRaf(V600E) Mouse Monoclonal Antibody
    Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers
    Immunogen:   A synthetic peptide from the internal region of BRaf(V600E), human origin.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:100-1:5000 
    WB: 1:100-1:2000 
    IHC: 1:100-1:1000
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody. Purified His - tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged B-Raf( V600D ) (lane 3) , His - tagged B-Raf( V600A ) (lane 4) , His - tagged B-Raf( V600G ) (lane 5) , His - tagged B-Raf( V600K ) (lane 6), His - tagged B-Raf( V600R ) (lane 7), and His - tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat # 26039) .
    Immunofluorescence:  
    Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
    Immunohistochemistry:   
    Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.
    310936477332.png

    Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with

    anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with
    paraffin. Samples were then incubated with primary antibody (1:100) overnight at 4°C.
     A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.
    Allele specific PCR validated to be Negative for BRAF V600E.

    图片.png

    Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with

    anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with

    paraffin. Samples were then incubated with primary antibody (1:100) overnight at 4°C. 

    A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. 

    Allele specific PCR validated to be Negative for BRAF V600E.


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    Publications:
    1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
        Mod Pathol. 2013 Mar;26(3):414-20.
    2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
        Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
    3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
        human pathology. February 2015Volume 46, Issue 2, Pages 169–175
    4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
        human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
    5.  BRAF V600E Mutation-Specific Antibody: A Review

        Seminars in Diagnostic Pathology

    6.Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922

        Oncotarget. 2016 Aug 2; 7(31): 49597–49610.

    7.PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio

       Sci Rep. 2019; 9: 1032.

    8.Targeting of the MAPK and AKT pathways in conjunctival melanoma shows potential synergy

        Oncotarget. 2017 Aug 29; 8(35): 58021–58036

    9.Clinical, Histologic, and Immunophenotypic Features of Serrated Polyps in Patients With Inflammatory Bowel Disease

        Gastroenterology Res. 2018 Oct; 11(5): 355–360