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Antibodies for G Proteins (Active and Inactive)

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Anti RheB Mouse Monoclonal Antibody

Price$239.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26015
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    RheB [26015]
    [100 µL]


    Catalog Number:  26015
    Gene Symbol:  RHEB
    Description:   Anti RheB Mouse Monoclonal Antibody
    Background:   RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.
    Immunogen:   Recombinant full length protein of RheB.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:100-1:5000 
    WB: 1:100-1:2000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  RheB antibody recognizes RheB proteins of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of RheB proteins. HEK-293 whole-cell lysate was used for Western blot.


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    Publications:
    1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
        Cancer Res. 2014 Feb 15;74(4):1067-78
    2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
        PLoS One. 2013 Dec 31;8(12):e83941